- 124 on Queen Hotel & Spa
- 627 on King Bed and Breakfast
- A Lil' Bit of Eden Bed and Breakfast
- Abacot Hall Bed & Breakfast
- Antique Slumber Old Town Bed and Breakfast
- Apple Tree Historic B&B
- Arnica
- As You Like It Bed and Breakfast
- Best Western Colonel Butler Inn
- Blue Skies Bed & Breakfast
- Brockamour Manor Bed and Breakfast
- Canterbury Inn
- Casa Blanca Boutique Bed & Breakfast
- Explorer House Bed & Breakfast
- Gatekeeper's Retreat
- Globetrotters B&B
- Graystone Bed & Breakfast
- Green Oaks B&B
- Greenview Manor Luxury Bed & Breakfast
- Hilton Garden Inn Niagara-on-the-Lake
- Historic Lyons House
- Historic Wilson-Guy House
- Holiday Inn Express Niagara-On-The-Lake
- In Elegance
- Luisa's Suite Retreat
- Moffat Inn
- Oban Inn
- Orchid Inn and Ginger Restaurant
- Pillar and Post
- Prince of Wales
- Queen Regent Bed & Breakfast
- Queens Landing
- Residence & Conference Centre - Niagara-On-The-Lake
- Riverbend Inn and Vineyard
- School House Bed and Breakfast C.1859
- Serendipity Bed and Breakfast
- South Landing Inn
- The Charles Hotel
- Via Veneto - Old Town
- Vineyard Villa
- WeatherPine Inn
- White Oaks Resort & Spa
- Williams Gate Bed & Breakfast Private Suites
- Woodbourne Inn